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Image Search Results
Journal: iScience
Article Title: Methods to separate nuclear soluble fractions reflecting localizations in living cells
doi: 10.1016/j.isci.2021.103503
Figure Lengend Snippet: Key resources table
Article Snippet: The antibodies used in this study were as follows: mAb414 ( Davis and Blobel, 1986 ) (902902; BioLegend) at 1:500 for IF, QE5 ( Panté et al., 1994 ) (ab24700; Abcam) at 1:100 for IF, RL2 ( Holt et al., 1987 ; Snow et al., 1987 ) (NB300-524; Novus biologicals) at 1:200 for IF, mouse anti-GAPDH (sc-32233; Santa Cruz Biotechnology) at 1:100 for IF and 1:1000 for WB, mouse anti-α-tubulin (T9026; Merck) at 1:500 for IF and 1:1000 for WB, goat anti-RanBP1 (ab17034; Abcam) at 1:200 for IF and 1:1000 for WB, mouse anti-Lamin A/C (sc-7292; Santa Cruz Biotechnology) at 1:200 for both IF and WB, rabbit anti-Histone H3 (ab1791; Abcam) at 1:200 for IF and 1:5000 for WB, goat anti-RCC1 (sc-1162; Santa Cruz Biotechnology) at 1:200 for both IF and WB, mouse anti-PCNA (610665; BD Biosciences) at 1:2000 for WB, mouse anti-PCNA (sc-56; Santa Cruz Biotechnology) at 1:50 for IF, rabbit anti-RanBP3 (NB100-74647; Novus biologicals) at 1:100 for IF and 1:1000 for WB, mouse anti- profilin 1 (PFN1) (67390-1-Ig; Proteintech) at 1:100 for IF and 1:2000 for WB,
Techniques: Recombinant, Protease Inhibitor, Plasmid Preparation, Magnetic Beads, Bicinchoninic Acid Protein Assay, Multiplex sample analysis, Multiplex Assay, Extraction, Software, Microscopy